+34 620 10 75 37info@nanbiosis.com

Services

Services

U3-S01. Synthesis of peptides and characterisation (On-site&Remote) OUTSTANDING

Synthesis of peptides at various different scales (100 mg to grams) and Purification and characterisation using HPCL and HPLC -MS .

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U2-S01. Scientific and technical support (Remote)

Scientific and technical support and advice on immunogen design, immunoreagent synthesis and production, antibody production and immunoassay design and development.

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U1-S05. Training courses in protein Production (On-site&Remote)

e.g. Course on “Strategies for optimization of recombinant protein production”)

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U1-S06. Criopreservation (On-site&Remote)

Criopreservation of Proteins, Plasmids, etc

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U1-S04. Consultancy in protein production (On-site&Remote)

In all matters dealing with protein production, expresion systems, etc, according to the final application of the product.

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U1-S03: Proteins purification (On-site&Remote) OUTSTANDING

  • Identification of the most appropriate strategy for purification, given the physical and chemical properties, opting either for purification modules or, in the case of fusion proteins, elements which enable purification by affinity.
  • Purification (downstream processing) using FPLC chromatography and/or tangential filtration.
  • Quality control of the purified protein ( molecular weight and purity by SDS-PAGE, biological activity, conformation by FTIR and DLS.
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U1-S01. Molecular cloning (On-site&Remote) OUTSTANDING

  • Identification of the most appropriate system, selecting between Escherichia coli, insect cells (with expression systems based on infection with Baculovirus) and mammalian cells, according to the characteristics of the desired protein in terms of its size, modifications, etc.
  • Cloning of the gene that codifies for the protein of interest in the chosen expression vector; selection of the positive genes and their characterization by sequencing.
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